Part:BBa_K4759050:Design

GFP11-linker-OleP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 112
  Illegal AgeI site found at 210
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Connecting GFP11 to the N terminus of Olep by linker and GFP1-10 to other proteins, and the interacting protein will bring GFP11 to GFP1-10 close to the fluorescence, the stronger the interaction, we screened the two proteins with the strongest interaction force by bimolecular fluorescence.

Source

fusion tag